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| Registros recuperados: 11 | |
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| d'Azevedo,Pedro Alves; Dias,Cícero Armídio Gomes; Lemos,Sibele Krebs; Bittencourt,José Augusto Ferreira; Teixeira,Lúcia Martins. |
| Resistance to several classes of antimicrobial agents is a remarkable characteristic of enterococcal strains increasingly reported worldwide. Information about strains isolated in the southern region of Brazil is still limited. In this study, a total of 455 consecutive enterococcal isolates recovered from patients living in Porto Alegre, Brazil, were identified at species level and evaluated for their antimicrobial susceptibilities by agar diffusion testing. The most frequent species was E. faecalis (92.8%), followed by E. faecium (2.9%), E. gallinarum (1.5%), E. avium (1.1%), E. hirae (0.7%), E. casseliflavus (0.4%), E. durans (0.4%), and E. raffinosus (0.2%). According to the results of disk tests 62.0% of the strains were resistant to tetracycline,... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcal; Antimicrobial susceptibility; Resistance. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822004000200005 |
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| Perez,Leandro Reus Rodrigues; d'Azevedo,Pedro Alves. |
| In this study, we determined the accuracy of phenotypic tests (cefoxitin agar dilution, 30µg-cefoxitin and 1µg-oxacillin disks) to detect the oxacillin resistance/mecA gene among coagulase-negative staphylococci (CoNS) isolates. The presence of the mecA gene was detected by PCR technique (gold standard). A total of 176 CoNS isolates from blood of hospitalized patients were evaluated. Of these, 138 (78.4%) harbored the mecA gene. Using 30µg-cefoxitin and 1µg-oxacillin disks we obtained 100 and 98.3% accuracy, respectively. In addition, when cefoxitin was used as marker in an agar dilution method, the higher accuracy (99.4%) was established with 8mg cefoxitin per mL breakpoint. Thus, despite of the agar dilution method using cefoxitin as a marker not being... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Oxacillin; Cefoxitin; CoNS. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000300009 |
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| Secchi,Carina; Antunes,Ana Lúcia Souza; Perez,Leandro Reus Rodrigues; Cantarelli,Vlademir Vicente; d'Azevedo,Pedro Alves. |
| The NCCLS (2004) presented a new methodology to detect, by disk-diffusion agar, oxacillin-resistance using a cefoxitin disk. We identified coagulase-negative staphylococci (SCoN) to the species level and compared the use of cefoxitin disks (30 µg) with oxacillin disks (1 µg), agar dilution (minimum inhibitory concentration of oxacillin) and mecA gene detection in isolates of coagulase-negative bacteria other than Staphylococcus epidermidis (SCoNne). A total of 238 SCoNne was evaluated; oxacillin-resistance (the mecA gene) was detected in 71% of the isolates. All methods gave 100% sensitivity, based on presence of the mecA gene. The specificity of the cefoxitin disk was 100%, while the oxacillin disk gave a specificity of 91% and agar dilution oxacillin... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Coagulase-negative Staphylococcus; Cefoxitin; Oxacillin; MecA gene; Susceptibility diagnostic. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702008000400012 |
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| Silveira,Alessandro Conrado de Oliveira; Cunha,Gabriela Rosa da; Caierão,Juliana; Cordova,Caio Mauricio Mendes de; d'Azevedo,Pedro Alves. |
| ABSTRACTTo determine the epidemiological and molecular characteristics of 12 Staphylococcus aureus isolates presenting heteroresistance to vancomycin in laboratories of two cities in Santa Catarina, southern Brazil. Epidemiological data, including the city of isolation, health institution, and date of isolation were considered, as well as the associated clinical specimen. For molecular characterization, we analyzed the staphylococcal cassette chromosome types, the erm gene presence, and the genomic diversity of isolates using pulsed-field gel electrophoresis. The 12 isolates of S. aureus were previously confirmed as heteroresistance to vancomycin using the population analysis profile-area under curve. Regarding genetic variability, two clones were... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: MRSA; Small colony variants; Antimicrobial resistance. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702015000500466 |
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| d'Azevedo,Pedro Alves; Santiago,Kelly Aline de Souza; Furtado,Guilherme Henrique Campos; Xavier,Diego Batista; Pignatari,Antonio Carlos Campos; Titze-de-Almeida,Ricardo. |
| The reduction in time required to identify vancomycin-resistant enterococci (VRE) has gained increased importance during hospital outbreaks. In the present study, we implemented a laboratory protocol to speed up the VRE screening from rectal samples. The protocol combines a medium for selective VRE isolation (VREBAC®, Probac, São Paulo) and a multiplex PCR for detection and identification of vanA and vanB resistance genes. The screening performance was analyzed in 114 specimens collected from four intensive care units. The swabs were collected at two periods: (1) during a VRE outbreak (February 2006, n=83 patients) and (2) at the post-outbreak period, after adoption of infection control measures (June 2006, n=31 patients). Forty-one/83 VRE (49.4%) and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enterococcus; VRE; Vancomycin; Diagnostic; PCR. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702009000400010 |
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| Registros recuperados: 11 | |
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